11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Endocrinology
Abstract
The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active
glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1,
predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids.
11β-HSD1-deficient mice show altered inflammatory responses and are protected against
the adverse metabolic effects of a high-fat diet. However, the impact of 11β-HSD1 on
the composition of the gut microbiome has not previously been investigated. We used
high-throughput 16S rDNA amplicon sequencing to characterise the gut microbiome
of 11β-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a
cholesterol- and fat-enriched ‘Western’ diet. 11β-HSD1 deficiency significantly altered
the composition of the gut microbiome, and did so in a diet-specific manner. On a
Western diet, 11β-HSD1 deficiency increased the relative abundance of the family
Bacteroidaceae, and on a chow diet, it altered relative abundance of the family
Prevotellaceae. Our results demonstrate that (i) genetic effects on host–microbiome
interactions can depend upon diet and (ii) that alterations in the composition of the
gut microbiome may contribute to the aspects of the metabolic and/or inflammatory
phenotype observed with 11β-HSD1 deficiency.
Description
Journal Article
Keywords
Glucocorticoids, 11β-HSD, Diet, Gut microbiome
Citation
Johnson, J. S., Opiyo, M. N., Thomson, M., Gharbi, K., Seckl, J. R., Heger, A., & Chapman, K. E. (2017). 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet. Journal of Endocrinology 232(273–283). DOI: 10.1530/JOE-16-0578