Vitamin B12 and Coenzyme Q10 Ameliorated Alcohol Driven Impairment of Hematological Parameters, Inflammation, and Organ Damage in A Mouse Model

Abstract

Introduction Chronic alcohol consumption is associated with a myriad of negative physiological and biochemical changes in humans. Vitamin B12 and coenzyme Q10 (CoQ10) are novel antioxidants and anti-inflammatory agents. Purpose The objective of this study was to determine the impact of oral supplementation with vitamin B12 and CoQ10, in attenuating deleterious effects associated with alcohol exposure in Swiss albino mice. Methods Group one was normal control, the second group received 5 g/kg alcohol; the third group received 6 mg/kg b/w of vitamin B12 and 5 g/kg alcohol; the fourth group received 6 mg/kg b/w of vitamin B12, 200 mg/kg b/w CoQ10, and 5 g/ kg alcohol, the fifth group 200 mg/kg b/w of CoQ10 and 5 g/kg alcohol. Results Oral administration of vitamin B12 and CoQ10 alone or in combination significantly ameliorated alcohol-induced impairment of hematological parameters and stabilized alcohol-induced alteration of the lipid profile. Notably, administration of either vitamin B12 or CoQ10 significantly blocked alcohol-induced depletion of reduced glutathione levels. Furthermore, vitamin B12 and CoQ10 stabilized the levels of pro-inflammatory cytokines (TNF-α and IFN-γ) when administered alone or in combination. Remarkably, the administration of CoQ10 and vitamin B12 significantly attenuated alcohol-induced liver and kidney inflammation and pathology. Conclusion Administration of either vitamin B12 or CoQ10 alone or in combination can protect from the toxic effects of chronic alcohol exposure.