Immunization with a combination of Leishmania major lipophosphoglycan (LPG) and Phlebotomus duboscqi salivary gland lysates (SGLs) abrogates protective efect of LPG against L. major in BALB/c mice
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
African Journal of Health Sciences
Abstract
The use of vector-derived antigens has become widely acknowledged as a possible answer to vaccination against vector borne diseases. Sand fly saliva is one such vector-derivative that has been targeted for vaccine development, either alone or in combination with other antigens. Previous studies have suggested a synergistic protective effect accruing from a parasite-derived and vector-derived cocktail vaccine. This study sought to evaluate such a synergistic effect in a cocktail vaccine comprising Phlebotomus duboscqi salivary gland lysates (SGLs) mixed with L. major lipophosphoglycan (LPG). Mice were immunized subcutaneously with SGLs, LPG or a cocktail of the two. The immunizations were then boosted twice every 2 weeks, followed by a challenge with 105 L. major metacyclic promastigotes 2 weeks after the last boost. Lesion growth was monitored over 35 days using vernier calipers, and footpad parasitaemia determined by simple limiting dilution assay. Immunizations with LPG alone gave effective protection against L. major infection (P < 0.05) compared to controls, whereas
SGLs, and the LPG + SGLs cocktail failed to protect. This study did not demonstrate a protective synergistic effect accruing from LPG + SGLs cocktail vaccine, and suggests a need to evaluate the effects of saliva in vaccinations with other Leishmanial antigens.
Description
Journal Article
Keywords
vaccination, vector borne diseases, Sand fly saliva
Citation
Karanja, R., Ingonga, J., Mwangi, M., Mwala, D., Lugalia, R., Magambo, J., & Tonui, , W. K. (2011). Immunization with a combination of Leishmania major lipophosphoglycan (LPG) and Phlebotomus duboscqi salivary gland lysates (SGLs) abrogates protective efect of LPG against L. major in BALB/c mice. African Journal of Health Sciences