Efficacy Of Combination Therapy Using Extracts Of Aloe Secundiflora Eng L. And Callistemon Citrinus William C. In Leishmania Major Infected Balb/C Mice

Simple item page
dc.contributor.authorNdeti, Cosmas
dc.contributor.authorChristine, Kituyi
dc.contributor.authorNdirangu, M.
dc.contributor.authorChimbevo, Lenny
dc.contributor.authorAnjili, Christopher
dc.contributor.authorBarasa, Mustafa
dc.contributor.authorOkendo, Javan Ochieng
dc.contributor.authorKinuthia, Geoffrey K.
dc.contributor.authorMaina, Esther
dc.contributor.authorNyambati, V. C. S.
dc.date.accessioned2024-05-14T09:11:52Z
dc.date.available2024-05-14T09:11:52Z
dc.date.issued2016-03
dc.descriptionJournal Article
dc.description.abstractBackground: Leishmania major causes cutaneous leishmaniasis which leads to painful skin sores in humans. In the current study, efficacy of combination therapy of A. secundiflora and C. citrinus against L. major infected mice treated intra-peritoneally and orally was studied. Pentostam administered intra-peritoneally and phosphate buffered saline intra-peritoneally and orally were used as a controls. Objective: To determine the efficacy of combined therapy of C. citrinus and A. secundiflora extracts in Leishmania major infected BALB/c mice. Design: Experimental-Laboratory based study Setting: Kenya Medical Research Institute (Leishmania Department) Subjects: Eight weeks Male BALB/c Mice Results: The minimum inhibitory concentration (MICs) of aqueous extracts of A. secundiflora (A), and C. citrinus (B) were 2 mg/ml and 5 mg/ml respectively while the IC50 for the same extracts were 467.09μg/ml and 457.88μg/ml respectively. The combination of these extracts at ratio (1:1) supported minimal growth of L. major promastigotes and had IC50 of 58.45μg/ml as compared to MICs of 12.50μg/ml for Pentostam. The combination therapy had Infection rate (IR) of 19% and MI of 52.81% compared to Pentostam (IR=21% and MI=11.64%). The combination therapy reduced the footpad lesion size significantly (P < 0.05) just like the Pentostam control drug and no significant nitric oxide was stimulated. The oral and intra-peritoneal combination treatment reduced spleen amastigotes in mice by 73.46% and 78.12% corresponding to total LDUs of 10.87±0.64 and 8.96±0.82 respectively compared to Pentostam at 94.58% and LDU of 2.22±0.13. The difference between efficacy of Pentostam and that of combined extracts was almost significant (t= 2.653, P= 0.057). Conclusion: The combination therapy was active against L. major parasite, reduced lesion size significantly and did not prevent visceralisation but reduced spleen parasite load significantly.
dc.identifier.citationNDETI C. M., KITUYI C., NDIRANGU M., INGONGA J., CHIMBEVO M. L., OCHIENG J. O., BARASA M., KINUTHIA G., MAINA E. M., NYAMBATI V. C. S. and ANJILI C. O. (2016): Efficacy Of Combination Therapy Using Extracts Of Aloe Secundiflora Eng L. And Callistemon Citrinus William C. In Leishmania Major Infected Balb/C Mice. East African Medical Journal
dc.identifier.urihttps://repository.daystar.ac.ke/handle/123456789/4623
dc.language.isoen
dc.publisherEast African Medical Journal
dc.relation.ispartofseriesVol. 93 No. 3
dc.subjectLeishmania major
dc.subjectCALLISTEMON CITRINUS
dc.subjectALOE SECUNDIFLORA
dc.titleEfficacy Of Combination Therapy Using Extracts Of Aloe Secundiflora Eng L. And Callistemon Citrinus William C. In Leishmania Major Infected Balb/C Mice
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Efficacy of combination therapy using extracts of Aloe secundiflora Eng L and Callistemon citrinus William C. in Leishmania major infected BALBc mice.pdf
Size:
340.33 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles