Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major

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dc.contributor.authorNgure, Peter
dc.contributor.authorNg’ang’a, Zipporah
dc.contributor.authorKimutai, Albert
dc.contributor.authorKepha, Stella
dc.contributor.authorMong’are, Samuel
dc.contributor.authorIngonga, Johnnie
dc.contributor.authorTonui, Willy
dc.date.accessioned2024-05-02T08:28:10Z
dc.date.available2024-05-02T08:28:10Z
dc.date.issued2014-01-18
dc.description.abstractIntroduction: To determine the immunostimulatory potential of crude extracts of Warburgia ugandensis subsp. ugandensis with a soluble leishmanial antigen in vaccinating BALB/c mice. Methods: Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble leishmania antigens (SLA) alone, hexane, ethyl acetate, and dichloromethane extract co-administered with SLA. Unvaccinated mice formed the control group. The induction of cell-mediated immunity following vaccination was determined by measuring in vitro lymphocyte proliferation and the production of interleukin (IL)-4 and gamma interferon (IFN-γ) determined by flow cytometry. Protection against L. major was determined by quantifying parasite burdens in L. major infected footpads using a limiting dilution assay and by measuring lesion sizes of the infected footpad compared to the contralateral uninfected footpad. Results: On vaccination with extracts of W. ugandensis subsp. ugandensis alone or as adjuvants when used in combination with Leishmania antigens, the hexane extract and the dichloromethane extract plus SLA stimulated moderate production of IFN-γ and low levels of IL-4.These mice were partially protected from cutaneous leishmaniasis as shown by the slow development of lesions and comparatively less parasite burdens. Conclusion: These data suggest that extracts of W. ugandensis subsp. ugandensis are suitable adjuvants for Leishmania vaccines. However, since W. ugandensis subsp. ugandensis has been shown to be effective against Leishmania parasites in vitro and in vivo, further studies ought to be conducted to determine its immunochemotherapeutic potential when co-administered with a soluble leishmanial antigen in vaccinating BALB/c mice.
dc.description.sponsorshipDaystar University, Kenya, Jomo Kenyatta University of Agriculture and Technology, Kabianga University, Kenya, Kenya Medical Training College, Kenya, Kenya Medical Research Institute, Kenya
dc.identifier.citationPeter Ngure, Zipporah Ng’ang’a, Albert Kimutai, Stella Kepha, Samuel Mong’are, Johnnie Ingonga, Willy Tonui. Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major. Pan Afr Med J. 2014;17(Supp 1):15
dc.identifier.issn1937-8688.
dc.identifier.urihttps://repository.daystar.ac.ke/handle/123456789/4370
dc.language.isoen
dc.publisherPan African Medical Journal.
dc.relation.ispartofseriesPan African Medical Journal. 2014;17(Supp 1):15
dc.subjectLeishmaniasis
dc.subjectImmunostimulatory responses
dc.subjectWarburgia ugandensis
dc.subjectCytokines
dc.titleImmunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
dc.typeArticle

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